Does triggering ingestion using Run Now affect the scheduled ingestion frequency of the Pipeline?

Manually triggering the ingestion using Run Now option does not affect the scheduled ingestion for the Object. The ingestion occurs as per the frequency set for the Pipeline.

Suppose you create a Pipeline at 3 PM (UTC) and set 1 Hour as the ingestion frequency. If you trigger ingestion using Run Now at 3:15 PM, the Events are ingested once and the next ingestion happens at 4 PM, as per the defined schedule. It is not moved to 4:15 PM. Thus, the Events are ingested at 3:15 PM, 4 PM, 5 PM, and so on.

Read Scheduling a Pipeline.